Evaluation of Commercial Diagnostic Assays for the Specific Detection of Avian Influenza A (H7N9) Virus RNA Using a Quality-Control Panel and Clinical Specimens in China
Identifieur interne : 000568 ( Main/Exploration ); précédent : 000567; suivant : 000569Evaluation of Commercial Diagnostic Assays for the Specific Detection of Avian Influenza A (H7N9) Virus RNA Using a Quality-Control Panel and Clinical Specimens in China
Auteurs : Dawei Shi [République populaire de Chine] ; Shu Shen [République populaire de Chine] ; Xingliang Fan [République populaire de Chine] ; Suhong Chen [République populaire de Chine] ; Dayan Wang [République populaire de Chine] ; Changgui Li [République populaire de Chine] ; Xing Wu [République populaire de Chine] ; Lili Li [République populaire de Chine] ; Dongting Bai [République populaire de Chine] ; Chuntao Zhang [République populaire de Chine] ; Junzhi Wang [République populaire de Chine]Source :
- PLoS ONE [ 1932-6203 ] ; 2015.
Abstract
A novel avian influenza A H7N9-subtype virus emerged in China in 2013 and threatened global public health. Commercial kits that specifically detect avian influenza A (H7N9) virus RNA are urgently required to prepare for the emergence and potential pandemic of this novel influenza virus. The safety and effectiveness of three commercial molecular diagnostic assays were evaluated using a quality-control panel and clinical specimens collected from over 90 patients with confirmed avian influenza A (H7N9) virus infections. The analytical performance evaluation showed that diverse influenza H7N9 viruses can be detected with high within- and between-lot reproducibility and without cross-reactivity to other influenza viruses (H1N1 pdm09, seasonal H1N1, H3N2, H5N1 and influenza B). The detection limit of all the commercial assays was 2.83 Log10 copies/μl [0.7 Log10TCID50/mL of avian influenza A (H7N9) virus strain A/Zhejiang/DTID-ZJU01/2013], which is comparable to the method recommended by the World Health Organization (WHO). In addition, using a WHO-Chinese National Influenza Center (CNIC) method as a reference for clinical evaluation, positive agreement of more than 98% was determined for all of the commercial kits, while negative agreement of more than 99% was observed. In conclusion, our findings provide comprehensive evidence for the high performance of three commercial diagnostic assays and suggest the application of these assays as rapid and effective diagnostic tools for avian influenza A (H7N9) virus in the routine clinical practice of medical laboratories.
Url:
DOI: 10.1371/journal.pone.0137862
PubMed: 26361351
PubMed Central: 4567293
Affiliations:
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Institutes for Food and Drug Control, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Shen, Shu" sort="Shen, Shu" uniqKey="Shen S" first="Shu" last="Shen">Shu Shen</name><affiliation wicri:level="3"><nlm:aff id="aff001"><addr-line>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Fan, Xingliang" sort="Fan, Xingliang" uniqKey="Fan X" first="Xingliang" last="Fan">Xingliang Fan</name><affiliation wicri:level="3"><nlm:aff id="aff001"><addr-line>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff002"><addr-line>WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chen, Suhong" sort="Chen, Suhong" uniqKey="Chen S" first="Suhong" last="Chen">Suhong Chen</name><affiliation wicri:level="3"><nlm:aff id="aff003"><addr-line>Beijing Institute of Radiation Medicine, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Institute of Radiation Medicine, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Dayan" sort="Wang, Dayan" uniqKey="Wang D" first="Dayan" last="Wang">Dayan Wang</name><affiliation wicri:level="3"><nlm:aff id="aff004"><addr-line>Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff005"><addr-line>WHO Collaborating Center for Reference and Research on Influenza, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>WHO Collaborating Center for Reference and Research on Influenza, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Changgui" sort="Li, Changgui" uniqKey="Li C" first="Changgui" last="Li">Changgui Li</name><affiliation wicri:level="3"><nlm:aff id="aff001"><addr-line>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff002"><addr-line>WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wu, Xing" sort="Wu, Xing" uniqKey="Wu X" first="Xing" last="Wu">Xing Wu</name><affiliation wicri:level="3"><nlm:aff id="aff001"><addr-line>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff002"><addr-line>WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Lili" sort="Li, Lili" uniqKey="Li L" first="Lili" last="Li">Lili Li</name><affiliation wicri:level="3"><nlm:aff id="aff001"><addr-line>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Bai, Dongting" sort="Bai, Dongting" uniqKey="Bai D" first="Dongting" last="Bai">Dongting Bai</name><affiliation wicri:level="3"><nlm:aff id="aff001"><addr-line>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Chuntao" sort="Zhang, Chuntao" uniqKey="Zhang C" first="Chuntao" last="Zhang">Chuntao Zhang</name><affiliation wicri:level="3"><nlm:aff id="aff001"><addr-line>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Junzhi" sort="Wang, Junzhi" uniqKey="Wang J" first="Junzhi" last="Wang">Junzhi Wang</name><affiliation wicri:level="3"><nlm:aff id="aff001"><addr-line>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff002"><addr-line>WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, People’s Republic of China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A novel avian influenza A H7N9-subtype virus emerged in China in 2013 and threatened global public health. Commercial kits that specifically detect avian influenza A (H7N9) virus RNA are urgently required to prepare for the emergence and potential pandemic of this novel influenza virus. The safety and effectiveness of three commercial molecular diagnostic assays were evaluated using a quality-control panel and clinical specimens collected from over 90 patients with confirmed avian influenza A (H7N9) virus infections. The analytical performance evaluation showed that diverse influenza H7N9 viruses can be detected with high within- and between-lot reproducibility and without cross-reactivity to other influenza viruses (H1N1 pdm09, seasonal H1N1, H3N2, H5N1 and influenza B). The detection limit of all the commercial assays was 2.83 Log<sub>10</sub> copies/μl [0.7 Log<sub>10</sub>TCID<sub>50</sub>/mL of avian influenza A (H7N9) virus strain A/Zhejiang/DTID-ZJU01/2013], which is comparable to the method recommended by the World Health Organization (WHO). In addition, using a WHO-Chinese National Influenza Center (CNIC) method as a reference for clinical evaluation, positive agreement of more than 98% was determined for all of the commercial kits, while negative agreement of more than 99% was observed. In conclusion, our findings provide comprehensive evidence for the high performance of three commercial diagnostic assays and suggest the application of these assays as rapid and effective diagnostic tools for avian influenza A (H7N9) virus in the routine clinical practice of medical laboratories.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Gao, R" uniqKey="Gao R">R Gao</name></author><author><name sortKey="Cao, B" uniqKey="Cao B">B Cao</name></author><author><name sortKey="Hu, Y" uniqKey="Hu Y">Y Hu</name></author><author><name sortKey="Feng, Z" uniqKey="Feng Z">Z Feng</name></author><author><name sortKey="Wang, D" uniqKey="Wang D">D Wang</name></author><author><name sortKey="Hu, W" uniqKey="Hu W">W Hu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, D" uniqKey="Liu D">D Liu</name></author><author><name sortKey="Shi, W" uniqKey="Shi W">W Shi</name></author><author><name sortKey="Shi, Y" uniqKey="Shi Y">Y Shi</name></author><author><name sortKey="Wang, D" uniqKey="Wang D">D Wang</name></author><author><name sortKey="Xiao, H" uniqKey="Xiao H">H Xiao</name></author><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Watanabe, T" uniqKey="Watanabe T">T Watanabe</name></author><author><name sortKey="Kiso, M" uniqKey="Kiso M">M Kiso</name></author><author><name sortKey="Fukuyama, S" uniqKey="Fukuyama S">S Fukuyama</name></author><author><name sortKey="Nakajima, N" uniqKey="Nakajima N">N Nakajima</name></author><author><name sortKey="Imai, M" uniqKey="Imai M">M Imai</name></author><author><name sortKey="Yamada, S" uniqKey="Yamada S">S Yamada</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Q" uniqKey="Zhang Q">Q Zhang</name></author><author><name sortKey="Shi, J" uniqKey="Shi J">J Shi</name></author><author><name sortKey="Deng, G" uniqKey="Deng G">G Deng</name></author><author><name sortKey="Guo, J" uniqKey="Guo J">J Guo</name></author><author><name sortKey="Zeng, X" uniqKey="Zeng X">X Zeng</name></author><author><name sortKey="He, X" uniqKey="He X">X He</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, H" uniqKey="Zhu H">H Zhu</name></author><author><name sortKey="Wang, D" uniqKey="Wang D">D Wang</name></author><author><name sortKey="Kelvin, Dj" uniqKey="Kelvin D">DJ Kelvin</name></author><author><name sortKey="Li, L" uniqKey="Li L">L Li</name></author><author><name sortKey="Zheng, Z" uniqKey="Zheng Z">Z Zheng</name></author><author><name sortKey="Yoon, Sw" uniqKey="Yoon S">SW Yoon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name></author><author><name sortKey="Zhou, L" uniqKey="Zhou L">L Zhou</name></author><author><name sortKey="Zhou, M" uniqKey="Zhou M">M Zhou</name></author><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z Chen</name></author><author><name sortKey="Li, F" uniqKey="Li F">F Li</name></author><author><name sortKey="Wu, H" uniqKey="Wu H">H Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiao, Xc" uniqKey="Xiao X">XC Xiao</name></author><author><name sortKey="Li, Kb" uniqKey="Li K">KB Li</name></author><author><name sortKey="Chen, Zq" uniqKey="Chen Z">ZQ Chen</name></author><author><name sortKey="Di, B" uniqKey="Di B">B Di</name></author><author><name sortKey="Yang, Zc" uniqKey="Yang Z">ZC Yang</name></author><author><name sortKey="Yuan, J" uniqKey="Yuan J">J Yuan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, Y" uniqKey="Ge Y">Y Ge</name></author><author><name sortKey="Wu, B" uniqKey="Wu B">B Wu</name></author><author><name sortKey="Qi, X" uniqKey="Qi X">X Qi</name></author><author><name sortKey="Zhao, K" uniqKey="Zhao K">K Zhao</name></author><author><name sortKey="Guo, X" uniqKey="Guo X">X Guo</name></author><author><name sortKey="Zhu, Y" uniqKey="Zhu Y">Y Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kang, X" uniqKey="Kang X">X Kang</name></author><author><name sortKey="Wu, W" uniqKey="Wu W">W Wu</name></author><author><name sortKey="Zhang, C" uniqKey="Zhang C">C Zhang</name></author><author><name sortKey="Liu, L" uniqKey="Liu L">L Liu</name></author><author><name sortKey="Feng, H" uniqKey="Feng H">H Feng</name></author><author><name sortKey="Xu, L" uniqKey="Xu L">L Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Wu, T" uniqKey="Wu T">T Wu</name></author><author><name sortKey="Qi, X" uniqKey="Qi X">X Qi</name></author><author><name sortKey="Ge, Y" uniqKey="Ge Y">Y Ge</name></author><author><name sortKey="Guo, X" uniqKey="Guo X">X Guo</name></author><author><name sortKey="Wu, B" uniqKey="Wu B">B Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name></author><author><name sortKey="Nian, Qg" uniqKey="Nian Q">QG Nian</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Hu, Y" uniqKey="Hu Y">Y Hu</name></author><author><name sortKey="Li, Xf" uniqKey="Li X">XF Li</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name></author><author><name sortKey="Feng, Y" uniqKey="Feng Y">Y Feng</name></author><author><name sortKey="Hu, D" uniqKey="Hu D">D Hu</name></author><author><name sortKey="Lv, H" uniqKey="Lv H">H Lv</name></author><author><name sortKey="Zhu, J" uniqKey="Zhu J">J Zhu</name></author><author><name sortKey="Cao, M" uniqKey="Cao M">M Cao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, Z" uniqKey="Zhu Z">Z Zhu</name></author><author><name sortKey="Fan, H" uniqKey="Fan H">H Fan</name></author><author><name sortKey="Qi, X" uniqKey="Qi X">X Qi</name></author><author><name sortKey="Qi, Y" uniqKey="Qi Y">Y Qi</name></author><author><name sortKey="Shi, Z" uniqKey="Shi Z">Z Shi</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, E" uniqKey="Chen E">E Chen</name></author><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name></author><author><name sortKey="Fu, L" uniqKey="Fu L">L Fu</name></author><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z Chen</name></author><author><name sortKey="Gong, Z" uniqKey="Gong Z">Z Gong</name></author><author><name sortKey="Mao, H" uniqKey="Mao H">H Mao</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name></author><author><name sortKey="Zhou, L" uniqKey="Zhou L">L Zhou</name></author><author><name sortKey="Zhou, M" uniqKey="Zhou M">M Zhou</name></author><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z Chen</name></author><author><name sortKey="Li, F" uniqKey="Li F">F Li</name></author><author><name sortKey="Wu, H" uniqKey="Wu H">H Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, S" uniqKey="Wang S">S Wang</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author><author><name sortKey="Chen, S" uniqKey="Chen S">S Chen</name></author><author><name sortKey="Guan, W" uniqKey="Guan W">W Guan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meng, Z" uniqKey="Meng Z">Z Meng</name></author><author><name sortKey="Han, R" uniqKey="Han R">R Han</name></author><author><name sortKey="Hu, Y" uniqKey="Hu Y">Y Hu</name></author><author><name sortKey="Yuan, Z" uniqKey="Yuan Z">Z Yuan</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author><author><name sortKey="Zhang, X" uniqKey="Zhang X">X Zhang</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name></author><author><name sortKey="Liang, W" uniqKey="Liang W">W Liang</name></author><author><name sortKey="Yang, S" uniqKey="Yang S">S Yang</name></author><author><name sortKey="Wu, N" uniqKey="Wu N">N Wu</name></author><author><name sortKey="Gao, H" uniqKey="Gao H">H Gao</name></author><author><name sortKey="Sheng, J" uniqKey="Sheng J">J Sheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hatchette, Tf" uniqKey="Hatchette T">TF Hatchette</name></author><author><name sortKey="Drews, Sj" uniqKey="Drews S">SJ Drews</name></author><author><name sortKey="Bastien, N" uniqKey="Bastien N">N Bastien</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="German, G" uniqKey="German G">G German</name></author><author><name sortKey="Antonishyn, N" uniqKey="Antonishyn N">N Antonishyn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kalthoff, D" uniqKey="Kalthoff D">D Kalthoff</name></author><author><name sortKey="Bogs, J" uniqKey="Bogs J">J Bogs</name></author><author><name sortKey="Harder, T" uniqKey="Harder T">T Harder</name></author><author><name sortKey="Grund, C" uniqKey="Grund C">C Grund</name></author><author><name sortKey="Pohlmann, A" uniqKey="Pohlmann A">A Pohlmann</name></author><author><name sortKey="Beer, M" uniqKey="Beer M">M Beer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nie, K" uniqKey="Nie K">K Nie</name></author><author><name sortKey="Zhao, X" uniqKey="Zhao X">X Zhao</name></author><author><name sortKey="Ding, X" uniqKey="Ding X">X Ding</name></author><author><name sortKey="Li, Xd" uniqKey="Li X">XD Li</name></author><author><name sortKey="Zou, Sm" uniqKey="Zou S">SM Zou</name></author><author><name sortKey="Guo, Jf" uniqKey="Guo J">JF Guo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chantratita, W" uniqKey="Chantratita W">W Chantratita</name></author><author><name sortKey="Sukasem, C" uniqKey="Sukasem C">C Sukasem</name></author><author><name sortKey="Kaewpongsri, S" uniqKey="Kaewpongsri S">S Kaewpongsri</name></author><author><name sortKey="Srichunrusami, C" uniqKey="Srichunrusami C">C Srichunrusami</name></author><author><name sortKey="Pairoj, W" uniqKey="Pairoj W">W Pairoj</name></author><author><name sortKey="Thitithanyanont, A" uniqKey="Thitithanyanont A">A Thitithanyanont</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V" uniqKey="Corman V">V Corman</name></author><author><name sortKey="Eickmann, M" uniqKey="Eickmann M">M Eickmann</name></author><author><name sortKey="Landt, O" uniqKey="Landt O">O Landt</name></author><author><name sortKey="Bleicker, T" uniqKey="Bleicker T">T Bleicker</name></author><author><name sortKey="Brunink, S" uniqKey="Brunink S">S Brunink</name></author><author><name sortKey="Eschbach Bludau, M" uniqKey="Eschbach Bludau M">M Eschbach-Bludau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cui, L" uniqKey="Cui L">L Cui</name></author><author><name sortKey="Liu, D" uniqKey="Liu D">D Liu</name></author><author><name sortKey="Shi, W" uniqKey="Shi W">W Shi</name></author><author><name sortKey="Pan, J" uniqKey="Pan J">J Pan</name></author><author><name sortKey="Qi, X" uniqKey="Qi X">X Qi</name></author><author><name sortKey="Li, X" uniqKey="Li X">X Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, J" uniqKey="Lu J">J Lu</name></author><author><name sortKey="Wu, J" uniqKey="Wu J">J Wu</name></author><author><name sortKey="Guan, D" uniqKey="Guan D">D Guan</name></author><author><name sortKey="Yi, L" uniqKey="Yi L">L Yi</name></author><author><name sortKey="Zeng, X" uniqKey="Zeng X">X Zeng</name></author><author><name sortKey="Zou, L" uniqKey="Zou L">L Zou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, D" uniqKey="Wang D">D Wang</name></author><author><name sortKey="Yang, L" uniqKey="Yang L">L Yang</name></author><author><name sortKey="Gao, R" uniqKey="Gao R">R Gao</name></author><author><name sortKey="Zhang, X" uniqKey="Zhang X">X Zhang</name></author><author><name sortKey="Tan, Y" uniqKey="Tan Y">Y Tan</name></author><author><name sortKey="Wu, A" uniqKey="Wu A">A Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name></author><author><name sortKey="Wang, D" uniqKey="Wang D">D Wang</name></author><author><name sortKey="Zheng, S" uniqKey="Zheng S">S Zheng</name></author><author><name sortKey="Shu, Y" uniqKey="Shu Y">Y Shu</name></author><author><name sortKey="Chen, W" uniqKey="Chen W">W Chen</name></author><author><name sortKey="Cui, D" uniqKey="Cui D">D Cui</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kang, K" uniqKey="Kang K">K Kang</name></author><author><name sortKey="Chen, L" uniqKey="Chen L">L Chen</name></author><author><name sortKey="Zhao, X" uniqKey="Zhao X">X Zhao</name></author><author><name sortKey="Qin, C" uniqKey="Qin C">C Qin</name></author><author><name sortKey="Zhan, Z" uniqKey="Zhan Z">Z Zhan</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Al Soud, Wa" uniqKey="Al Soud W">WA Al-Soud</name></author><author><name sortKey="Radstrom, P" uniqKey="Radstrom P">P Radstrom</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rossen, L" uniqKey="Rossen L">L Rossen</name></author><author><name sortKey="Norskov, P" uniqKey="Norskov P">P Norskov</name></author><author><name sortKey="Holmstrom, K" uniqKey="Holmstrom K">K Holmstrom</name></author><author><name sortKey="Rasmussen, Of" uniqKey="Rasmussen O">OF Rasmussen</name></author></analytic></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Shi, Dawei" sort="Shi, Dawei" uniqKey="Shi D" first="Dawei" last="Shi">Dawei Shi</name></noRegion><name sortKey="Bai, Dongting" sort="Bai, Dongting" uniqKey="Bai D" first="Dongting" last="Bai">Dongting Bai</name><name sortKey="Chen, Suhong" sort="Chen, Suhong" uniqKey="Chen S" first="Suhong" last="Chen">Suhong Chen</name><name sortKey="Fan, Xingliang" sort="Fan, Xingliang" uniqKey="Fan X" first="Xingliang" last="Fan">Xingliang Fan</name><name sortKey="Fan, Xingliang" sort="Fan, Xingliang" uniqKey="Fan X" first="Xingliang" last="Fan">Xingliang Fan</name><name sortKey="Li, Changgui" sort="Li, Changgui" uniqKey="Li C" first="Changgui" last="Li">Changgui Li</name><name sortKey="Li, Changgui" sort="Li, Changgui" uniqKey="Li C" first="Changgui" last="Li">Changgui Li</name><name sortKey="Li, Lili" sort="Li, Lili" uniqKey="Li L" first="Lili" last="Li">Lili Li</name><name sortKey="Shen, Shu" sort="Shen, Shu" uniqKey="Shen S" first="Shu" last="Shen">Shu Shen</name><name sortKey="Wang, Dayan" sort="Wang, Dayan" uniqKey="Wang D" first="Dayan" last="Wang">Dayan Wang</name><name sortKey="Wang, Dayan" sort="Wang, Dayan" uniqKey="Wang D" first="Dayan" last="Wang">Dayan Wang</name><name sortKey="Wang, Junzhi" sort="Wang, Junzhi" uniqKey="Wang J" first="Junzhi" last="Wang">Junzhi Wang</name><name sortKey="Wang, Junzhi" sort="Wang, Junzhi" uniqKey="Wang J" first="Junzhi" last="Wang">Junzhi Wang</name><name sortKey="Wu, Xing" sort="Wu, Xing" uniqKey="Wu X" first="Xing" last="Wu">Xing Wu</name><name sortKey="Wu, Xing" sort="Wu, Xing" uniqKey="Wu X" first="Xing" last="Wu">Xing Wu</name><name sortKey="Zhang, Chuntao" sort="Zhang, Chuntao" uniqKey="Zhang C" first="Chuntao" last="Zhang">Chuntao Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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